Pediatric tumor unit

Characterization of new target genes of the EWSR1-FLI1 oncoprotein characteristic of Ewing sarcoma.

Ewing's sarcoma is a rare tumor that affects children and adolescents. These tumors are extremely aggressive. Thus, 40% of affected children and adolescents will die as a consequence of the tumor. These tumors are characterized by the presence of chromosomal translocations that result in the fusion of the EWSR1 gene with the FLI1 transcription factor, thus giving rise to a new chimeric transcription factor called EWSR1-FLI1 that plays a central role in the development of the tumor by altering the gene expression profile. The objective of this master's work will be to identify new target genes for EWSR1-FLI1 and their functional characterization. For this, cellular models of EWSR1-FLI1 silencing (shRNA and CRISPR / Cas9) and a variety of molecular and cellular biology techniques and animal models will be used. The ultimate goal is to identify genes and routes that can lead us to the development of new therapies.