Ingeniería Enzimática

MULTIENZYME PROCESSES WITH THERMOPHILE AND MESOPHYL ENZYMES: SELECTIVE HEATING OF THERMOPHILE ENZYMES

Thermophilic enzymes are very stable to heat inactivation, but need to work at moderately high temperatures. We propose a selective reduction of ketones catalyzed by a thermophilic alcohol dehydrogenase. As an auxiliary enzyme we will use a mesophilic enzyme: formate dehydrogenase.
We will immobilize magnetic nanoparticles (12 nm in diameter) (NPMs) inside macro-porous solids (with a diameter of 200 nm) and the thermophilic enzyme will be immobilized on the NPMs. The mesophilic enzyme will be immobilized on the surface of the macro-porous support. These hybrid supports are ideal for immobilization of enzymes
In this way we can keep the entire reactor at room temperature and we will only heat the NPMs and the enzyme immobilized on them by the effect of an alternating magnetic field (AMF).

We will study the immobilization of an alcohol dehydrogenase from Thermus thermophillus on MNPs and we will study their heating by the Magnetic Field. We will study different supports and different distributions of the NPMs in the supports