Dendritic Cell and Macrophage Immunobiology Laboratory

The proposed research project focuses on the study of the interaction between peritoneal macrophages and tumor cells during the first stages of peritoneal colorectal cancer metastasis. The experimental model is based on the intraperitoneal injection of mouse colorectal tumor-derived organoids (MTOs – mouse tumor organoids), a technique that is already being regularly used in our laboratory. The experimental work includes unfreezing/freezing and culture of colorectal tumor-derived organoids, sample preparation and analysis by Whole Mount Immunofluorescence technique and confocal microscopy, semi-fine and ultra-fine sections and analysis by high-resolution light microscopy and electron microscopy, sample preparation and analysis by cytocentrifugation and sample preparation and analysis by flow cytometry.