T cell physiopathology

SCIENTIFIC AREA
Inmunology
CENTER
Otro
VACANCIES
0
CONTACT E-MAIL
regueiro@med.ucm.es
DESCRIPTION OF THE OFFER

DESCRIPTION OF THE OFFER: Blood cells, especially erythrocytes, are protected from complement deposition and lysis by membrane regulators such as CD46/MCP. Somatic or congenital loss-of-function mutations in CD46 may cause atypical haemolytic uremic syndrome (aHUS). We believe aHUS patient-derived B cell lines could be useful as screening biosensors for the lytic activity of plasma samples with dysfunctional regulation of complement activation, for instance, due to factor H deficiency.

Aims and activities:

1)      To generate Epstein-Barr virus (EBV)-immortalized B cell lines from aHUS patients with CD46/MCP deficiency.

2)      To analyse C3/C3b deposition by flow cytometry on these EBV cell lines after incubation with factor H-deficient plasma.

3)      To validate and eventually commercialize these cell lines as biosensors of plasma C3 dysregulation in plasma samples from patients with complement disorders.

MASTER
Molecular Biomedicine
SUPERVISOR TFM
Anaïs Jiménez Reinoso