Cristalografía y Biología Estructural.
Protein Structure and Function
Instituto de Química Física "Rocasolano" CSIC (IQFR)
DESCRIPTION OF THE OFFER
Study of the enzyme ScIPK1 involved in inositol hexakisphosphate synthesis in yeasts.
The inositol phosphate kinases (IPKs) are a group of enzymes that regulate the levels of the inositol phosphates (IPs). This compounds are second messengers and essential metabolites present in eukaryotic organisms. In particular, inositol hexakisphosphate (IP6) participates in multiple events, especially those related to RNA (transport, editing, transcription, etc.). In turn, IP6 has been shown to play a significant role in apoptosis, cancer and nutrition. In the last decade, our group has been focused in the Structural Biology of the IPKs, and in particular to the study of the enzyme IPK1 responsible for the IP6 synthesis. Up to now we have obtained the structures of plant and mammalian IPK1 by Crystallography. However, we do not know the structure of the yeast enzyme, whose sequence exhibit marked differences from the two above. Since most of the IPK1 roles have been demonstrated in yeasts, knowledge of its structure would help to better understand its multiple functions.
In this work, the student will produce suitable DNA constructs of ScIPK1 and develop a protocol for its expression and purification, in order to try the protein crystallization. In case of success, the student would initiate in X-ray diffraction data collection and in protein structural solution techniques.
Biomolecules & Cell D.
Beatriz González Pérez