Epigenomic alterations in human carcinoma
Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT)
DESCRIPTION OF THE OFFER
Numerous scientific evidence suggests that epigenetic alterations (DNA methylation, covalent histone modifications, chromatin remodeling, and non-coding RNA) are frequently implicated in cancer. However, very little is known about the importance of such alterations in cancers such as head and neck squamous cell carcinoma (HNSCC). HNSCC is a major cancer, caused mainly by tobacco and / or alcohol abuse, or by human papillomavirus (HPV) infection. The TFM research theme will focus on how the mutations of KMT2D and KMT2C affect the HNSCC epigenome, both in the presence and absence of HPV infection. Both proteins would act as tumor suppressors that control the functioning of regulatory enhancer regions of the genome, and the expression of a new class of non-coding RNA called enhancer-derived RNAs (eRNAs). The project involve the use of HNSCC cell lines and state-of-the-art technologies such as CRISPR/Cas, massive RNA sequencing and chromatin immunoprecipitation (RNAseq, ChIP-seq).
Biomolecules & Cell D.