Unidad de Neuroinflamación

TFM1 (Eva Cano/Andrés Fernández): Cell culture, characterization and differentiation of pluripotent stem cells to microglial phenotype.

Induced pluripotent cells, reprogrammed from somatic cells (iPS) represent a very practical and versatile tool in the neurobiology field. The objectives of this project are:

1. Culture and characterization of pluripotent cells from different sources: mouse iPS cells (miPS), obtained from nuclefection with the plasmid pCAG -OSKG and embryonic stem cells already established.

2. Comparison of the Oct4 and Sox2 markers levels in stem cells of different origins by immunocytochemistry.

3. Comparison of differentiation potential of the different cell types: spontaneous differentiation through the formation of embryoid bodies and directed differentiation towards microglial phenotype.

4.- Characterization of these population by immunohistochemistry, flow cytometry and gene analysis using quantitative PCR.