Unidad de Neuroinflamación
Molecular and Cellular Neurobiology
Instituto de Salud Carlos III (ISC III)
DESCRIPTION OF THE OFFER
TFM1 (Eva Cano): Cell culture, characterization and differentiation of pluripotent stem cells to microglial phenotype.
Induced pluripotent cells, reprogrammed from somatic cells (iPS) represent a very practical and versatile tool in the neurobiology field. The objectives of this project are:
1. Culture and characterization of pluripotent cells from different sources: mouse iPS cells (miPS), obtained from nuclefection with the plasmid pCAG -OSKG and embryonic stem cells already established.
2. Comparison of the Oct4 and Sox2 markers levels in stem cells of different origins by immunocytochemistry.
3. Comparison of differentiation potential of the different cell types: spontaneous differentiation through the formation of embryoid bodies and directed differentiation towards microglial phenotype.
4.- Characterization of these population by immunohistochemistry, flow cytometry and gene analysis using quantitative PCR.
TFM2: (Andres Fernandez) Role of brain myeloid cells in the development of glioma: a therapeutic target to investigate.
1.-The student will implement an orthotopic murine model of GL261 glioma, which will allow us to study the immune system responses to gliomas. Macrophages / microglia in glioblastoma are directly related to malignancy, making isolation, phenotypic and functional characterization of great importance in the field of Neuro-oncology.
2. Optimize an isolation method of brain myeloid cells with anti-CD45/anti-CD11b antibodies coupled to magnetic beads (MACS technology).
3. Phenotypic analysis of gliomas associated myeloid cells: Flow cytometry analysis (FACS), phenotype analyzes by immunohistochemistry and gene analysis by quantitative RT-PCR.
Biomolecules & Cell D.
Andrés Fernández Gil