Pediatric tumor unit

Role of extracellular vesicles in the pathogenesis of Ewing's sarcoma

Ewing's sarcoma is a rare tumor that affects children and adolescents. These tumors are extremely aggressive. Thus, 40% of affected children and adolescents will die as a consequence of the tumor. The main objective of this master's work is to characterize the role of extracellular vesicles such as exosomes in the development of Ewing's sarcoma. In recent years there has been a growing interest in studying the role of these vesicles that can transport multiple bioactive molecules and alter the behavior of tumor cells. The role of these vesicles in the development of Ewing's sarcoma is not yet well understood. Different cell models of Ewing's sarcoma will be used to study the composition of these vesicles using proteomic and transcriptomic techniques, and their effect on the behavior of tumor cells will be studied using a variety of cellular and molecular biology techniques. The therapeutic potential of these vesicles will also be assessed in the context of Ewing's sarcoma.