Molecular Oncology Group
Instituto de Investigación Sanitaria Aragón (IIS Aragón)
DESCRIPTION OF THE OFFER
The Molecular Oncology Group at the Aragón Health Research Institute (IIS Aragón) is interested in a young motivated researcher that would like to join us to perform the Master Thesis (TFM).
The current focus of research in the lab is the development of non-invasive immunotargeted imaging tools for the diagnosis of brain tumors. This approach combines the target selectivity and specificity of antibodies and engineered fragments against a given tumor cell surface marker with the main characteristics (spatial resolution, sensitivity, quantifiable) of imaging techniques. The merge of the antibody-specificity with the high resolution and quantitative capabilities of PET makes the immuno-PET a “virtual biopsy”, comparable to conduct an in vivo integrated, quantifiable, 3D, full body immunohistochemistry allowing the diagnosis and monitoring of patients over time in a non-invasive manner.
The objective of the TFM is the generation of immunotargeted agents against ABCC3 for the diagnosis of glioblastoma. Applying bioinformatics analysis in patient databases we have determined that ABCC3 could be a good biomarker against glioblastoma (GBM) for its use in molecular imaging because its expression (mRNA) correlates with malignancy, resistance to chemotherapy and worse survival.
The TFM work will consist of the validation of ABCC3 as a biomarker in GBM and the generation of immuno-directed agents. First, we will validate the ABCC3 overexpression at the protein level in a collection of patient samples. These results will be correlated with tumor grade, survival and response to treatments. The candidate will generate gain and loss of function cellular models by using overexpression vectors and CRISPR/Cas9 technologies to determine the response to the most common treatments. Subsequently, nanobodies will be developed against ABCC3 for their evaluation in immunoPET. For this purpose, we will apply E. coli display and Phage display from gene libraries generated from lymphocytes upon camel immunization. The candidate will learn a range of tecnhiques including Molecular Biology (cloning, CRISPR/Cas9, qRTPCR, purification of nucleic acids and proteins, WB, PCR), cell biology (cultures, dose response assays, virus preparation, selection), immunohistochemistry and flow cytometry.
1.- Degree related to biological sciences finished or near finished.
2.- Good academic record.
3.- High motivation for research.
4.- Good English language level.
We are a new young research group which is located in the Centro de Investigación Biomédica de Aragón (CIBA, Zaragoza, Spain). We combine a series of molecular biology techniques, mouse models, genome wide screenings and cell biology approaches to perform our research.
Publications of the PI: https://www.ncbi.nlm.nih.gov/pubmed/?term=schuhmacher+aj
Information and application
To obtain more information about the position as well as to apply for it, please contact Alberto J. Schuhmacher (email@example.com) providing a detailed CV.
Alberto J. Schuhmacher, PhD.
Instituto de Investigación Sanitaria Aragón
Centro de Investigación Biomédica de Aragón (CIBA)
Avda. Juan Bosco 13, 50009 Zaragoza
Alberto Jiménez Schuhmacher