Centro Nacional de Investigaciones Oncológicas (CNIO)
DESCRIPTION OF THE OFFER
Applications are sought for a TFM to join the Molecular Cytogenetics Unit (led by Dr. Juan c Cigudosa) at the Spanish National Cancer Center (CNIO), Madrid, Spain.
Our laboratory focuses on the implementation of genome engineering technologies (including CRISPR-Cas9) to model human cancer aberrations in primary cells. Methodologies to reproduce tumour-associated chromosome aberrations are necessary to perform the functional identification and detailed characterisation of cancer genes. We have developed an efficient strategy, based on the CRISPR genome editing system, to generate human cell models bearing tumour specific chromosomal alterations (Nat Commun 2014), demonstrating that the in vitro modelling of tumour-initiating cells is feasible with CRISPR. We have optimised our CRISPR protocol to increase the efficiency of engineered chromosome aberrations in poorly transfectable human primary cells, reducing the labour and costs associated with single cell cloning and genotyping after cell modelling. We use multi-disciplinary approaches including gene editing, molecular biology, cell biology, molecular cytogenetics, and preclinical models in our studies. We are equipped with a state-of-the-art equipment to conduct our projects.
Raul Torres Ruiz